Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli | Pyrobaculum aerophilum |
General Stability | Organism |
---|---|
Zn2+ is not essential for structural stability of the enzyme | Pyrobaculum aerophilum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 20% decrease in activity | Pyrobaculum aerophilum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.4 | - |
3,4-dihydro-1(2H)-naphthalenone | 95°C, pH 7.5 | Pyrobaculum aerophilum |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Zn2+ | important for activity | Pyrobaculum aerophilum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
38580 | - |
x * 38580, calculated from sequence | Pyrobaculum aerophilum |
40000 | - |
x * 40000, SDS-PAGE | Pyrobaculum aerophilum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pyrobaculum aerophilum | Q8ZUP0 | - |
- |
Pyrobaculum aerophilum DSM 7523 | Q8ZUP0 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Pyrobaculum aerophilum |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
60°C, pH 7.5 | Pyrobaculum aerophilum |
50 | - |
95°C, pH 7.5 | Pyrobaculum aerophilum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+ | 3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone. The enzyme does not show activity on a large number of alcohols, aldehydes or ketones. It is active only with alpha-tetralone as substrate | Pyrobaculum aerophilum | 1,2,3,4-tetrahydronaphthalen-1-ol + NADP+ | - |
? | |
3,4-dihydro-1(2H)-naphthalenone + NADPH + H+ | 3,4-dihydro-1(2H)-naphthalenone i.e. alpha-tetralone. The enzyme does not show activity on a large number of alcohols, aldehydes or ketones. It is active only with alpha-tetralone as substrate | Pyrobaculum aerophilum DSM 7523 | 1,2,3,4-tetrahydronaphthalen-1-ol + NADP+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 40000, SDS-PAGE | Pyrobaculum aerophilum |
? | x * 38580, calculated from sequence | Pyrobaculum aerophilum |
Synonyms | Comment | Organism |
---|---|---|
PAE2687 | locus name | Pyrobaculum aerophilum |
PyAeADHII | - |
Pyrobaculum aerophilum |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
95 | - |
reaction rate increases with temperature up to 95°C | Pyrobaculum aerophilum |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1719 | - |
3,4-dihydro-1(2H)-naphthalenone | 95°C, pH 7.5 | Pyrobaculum aerophilum |